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KMID : 1007519980070010056
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Food Science and Biotechnology
1998 Volume.7 No. 1 p.56 ~ p.59
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Isolation and Identification of Tyrosinase Inhibitor from Galla Rhois
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Kim, Hyo Jin
Choi, Sang Won/Sapers, Gerald M
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Abstract
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Two hundred-five crude drug extracts were previously screened for their tyrosinase inhibitory activity. Among them, the 50% aqueous methanols extracts of Galla Rhois showed the potent inhibitory activity (IC_(50)=0.2 ㎎/mL). To isolate active compounds, the 50% aqueous methanol extracts was evaporated to a small volumn in vacuo, and then fractionated by the Diaion HP-20, Sephadex LH-20 column chromatography and repeated the preparative HPLC procedure. Three major phenolic compounds were isolated, and one of them exhibiting tyrosinase inhibitory activity stronger than L-ascorbic acid (IC_(50)=70 ㎍/mL) was identified as 1 2,3 4 6-penta-O-galloyl-β-D-glucose (IC_(50)=50 ㎍/mL) by UV, IR, ¹H/^(13)C-NMR, and FAB-MS spectroscopy. This compound showed a typical noncompetitive inhibition pattern against mushroom tyrosinase.
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KEYWORD
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